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Autoclave Safety

Caution: Autoclaves may cause serious burns

To Prevent Injury:

  • Loosen screw caps on bottles and tubes of liquids before autoclaving.
  • Check that chamber pressure has returned to zero before opening door.
  • Wear eye and face protection.
  • Stand behind door when opening it.
  • Slowly open door only a crack. Beware rush of steam.
  • Keep face away from door as it opens. Escaping steam may burn face.
  • Wait 5 minutes after opening door before removing liquids.
  • Liquids removed too soon may boil up and out of container, burning operator.